Since our founding in 1984, Catachem has developed a rich 20 year history in clinical chemistry as a manufacturer and distributor of in-vitro chemistry products and services for human, veterinary and environmental laboratories.

Catachem chemists helped develop many of the automated diagnostic chemistries widely in use today. Catachem created the DiscretePak™ and VetSpec™ line of reagents for human and veterinary automated analyzers.

Catachem offers FDA approved (510K) chemistries (liquid and dry) including enzymatic chemistries developed in conjunction with the published guidelines of IFCC and CDC.

Catachem has more than 500 applications for all types of analyzers including the Roche™, Hitachi™, Olympus™, AU Series™, RA Series™, Opera™, DAX™, Bayer™, Advia™, Synchron™, Beckman™, Abbott™ and many other analyzers.

Corporate headquarters, research and development, custom formulation laboratories, and manufacturing are located in Bridgeport, Connecticut. Catachem supplies leading laboratories and equipment manufacturers from distributors around the world.

PLASMA FREE HEMOGLOBIN REAGENT

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Plasma Free Hemoglobin in serum using manual or automated applications.

The Catachem colorimetric procedure for the determination of hemoglobin in plasma is based upon the peroxidase activity of hemoglobin. In this procedure hemoglobin activates the oxidation of 3.3’,5.5’-tetramethylbenzidine by hydrogen peroxide to form a chromogenic product with maximum absorption at 600nm. The increase of absorbance is directly proportional to the concentration of hemoglobin in the plasma sample. This procedure is simple and accurate and it is based on the work of Lijana, RC, and Williams, MC, and Standefer, J.C. and Vanderjagt, D.

Reagent Storage And Stability

Catachem Plasma Hemoglobin reagents are stored at 2-8°C. When stored as directed the reagents are stable until expiration date stated on the label.

Working Reagent Preparation

Catachem Plasma Hemoglobin reagents are packaged in ready-to-use form. No preparation is required. Upon opening, the Working Reagents are stable for at least 30 days stored at 2-8ºC and capped while not in use.

Method Performance Characteristics

Sensitivity: The sensitivity of this method is 0.010-0.012 absorbance units per mg/dL.

Linear Range: In this method there is no significant nonlinearity over the range of  0-75 mg/dL.

Precision: Within-run and ady-t-day precsion is summarized below.

PFH

Within-Run Precision

Total Precision

MEAN

SD

CV

SD

CV

Mg/dL

Mg/dL

%

mg/dL

%

5.0

0.710

16.70

25.0

0.714

2.99

45.0

1.08

2.29